2024

Campbell, S. L. & Christofk, H. R. Lessons learned from cancer metabolism for physiology and disease. Cold Spring Harbor Perspectives in Medicine (2024). doi:10.1101/cshperspect.a041554 Christofk, H., Metallo, C., Liu, G., Rabinowitz, J., Sparks, L., & James, D. (2024). Metabolic heterogeneity in humans. Cell, 187(15), 3821–3823. https://doi.org/10.1016/j.cell.2024.06.033

2023

Perez-Ramirez CA, Nakano H, Law RC, Matulionis N, Thompson J, Pfeiffer A, Park JO, Nakano A, Christofk HR. Atlas of fetal metabolism during mid-to-late gestation and diabetic pregnancy. 2023 Dec 8;S0092-8674(23)01228-X. doi: 10.1016/j.cell.2023.11.011.Tu WB, Christofk HR, Plath K. Nutrient regulation of development and cell fate decisions. Development. 2023 Oct 15;150(20):dev199961. doi: 10.1242/dev.199961.Wilde BR, Chakraborty N, Matulionis N, Hernandez S, Ueno D, Gee ME, Esplin ED, Ouyang K, Nykamp K, Shuch B, Christofk HR. FH variant pathogenicity promotes purine salvage pathway dependence in kidney cancer. Cancer Discov. 2023 May 31:CD-22-0874. doi: 10.1158/2159-8290.CD-22-0874.Cicchetto AC, Jacobson EC, Sunshine H, Wilde BR, Krall AS, Jarrett KE, Sedgeman L, Turner M, Plath K, Iruela-Arispe ML, de Aguiar Vallim TQ, Christofk HR. ZFP36-mediated mRNA decay regulates metabolism. Cell Rep. 2023 Apr 21;42(5):112411. doi: 10.1016/j.celrep.2023.112411.

2021

Mullen PJ, Garcia G Jr, Purkayastha A, Matulionis N, Schmid EW, Momcilovic M, Sen C, Langerman J, Ramaiah A, Shackelford DB, Damoiseaux R, French SW, Plath K, Gomperts BN, Arumugaswami V, Christofk HR. SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition. Nat Commun. 2021 Mar 25;12(1):1876. doi: 10.1038/s41467-021-22166-4. PMID: 33767183.Perez-Ramirez CA, Christofk HR. Challenges in Studying Stem Cell Metabolism. Cell Stem Cell. 2021 Mar 4;28(3):409-423. doi: 10.1016/j.stem.2021.02.016. PMID: 33667361.Krall AS, Mullen PJ, Surjono F, Momcilovic M, Schmid EW, Halbrook CJ, Thambundit A, Mittelman SD, Lyssiotis CA, Shackelford DB, Knott SRV, Christofk HR. Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth. Cell Metabolism. 2021 Feb 17:S1550-4131(21)00057-7. doi: 10.1016/j.cmet.2021.02.001.

2020

Blake R Wilde, Heather R Christofk. O-Mg! Lactate Drives Mg 2+ Mobilization. Molecular Cell. Dec, 2020. https://doi.org/10.1016/j.molcel.2020.11.023Shapira, Suzanne N., and Heather R. Christofk. 2020. Metabolic Regulation of Tissue Stem Cells. Trends in Cell Biology. Elsevier Ltd. doi: 10.1016/j.tcb.2020.04.004.

2019

Momcilovic M, Jones A, Bailey ST, Waldmann CM, Li R, Lee JT, Abdelhady G, Gomez A, Holloway T, Schmid E, Stout D, Fishbein MC, Stiles L, Dabir DV, Dubinett SM, Christofk H, Shirihai O, Koehler CM, Sadeghi S, Shackelford DB. In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer. Nature. 2019 Nov;575(7782):380-384.Thaker SK, Ch'ng J, Christofk HR. Viral hijacking of cellular metabolism. BMC Biol. 2019 Jul 18;17(1):59.Thaker SK, Chapa T, Garcia G Jr, Gong D, Schmid EW, Arumugaswami V, Sun R, Christofk HR. Differential Metabolic Reprogramming by Zika Virus Promotes Cell Death in Human versus Mosquito Cells. Cell Metab. 2019 Feb 16. pii: S1550-4131(19)30064-6.Flores A, Sandoval-Gonzalez S, Takahashi R, Krall A, Sathe L, Wei L, Radu C, Joly JH, Graham NA, Christofk HR, Lowry WE. Increased lactate dehydrogenase activity is dispensable in squamous carcinoma cells of origin. Nat Commun. 2019 Jan 9;10(1):91. doi: 10.1038/s41467-018-07857-9.

2018

William J. Sullivan, Peter J. Mullen, Ernst W. Schmid, Aimee Flores, Milica Momcilovic, Mark S. Sharpley, David Jelinek, Andrew E. Whiteley, Matthew B. Maxwell, Blake R. Wilde, Utpal Banerjee, Hilary A. Coller, David B. Shackelford, Daniel Braas, Donald E. Ayer, Thomas Q. de Aguiar Vallim, William E. Lowry, and Heather R. Christofk. Extracellular Matrix Remodeling Regulates Glucose Metabolism through TXNIP Destabilization. Cell. 175, 116, September 20, 2018.Jelinek D, Flores A, Uebelhoer M, Pasque V, Plath K, Iruela-Arispe ML, Christofk HR, Lowry WE, Coller HA. Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue. J Vis Exp. 2018 Jun 21;(136). doi: 10.3791/57760.Milica Momcilovic, Sean T. Bailey, Jason T. Lee, Michael C. Fishbein, Daniel Braas, James Go, Thomas G. Graeber, Francesco Parlati, Susan Demo, Rui Li, Tonya C. Walser, Michael Gricowski, Robert Shuman, Julio Ibarra, Deborah Fridman, Michael E. Phelps, Karam Badran, Maie St. John, Nicholas M. Bernthal, Noah Federman, Jane Yanagawa, Steven M. Dubinett, Saman Sadeghi, Heather R. Christofk, David B. Shackelford. The GSK3 Signaling Axis Regulates Adaptive Glutamine Metabolism in Lung Squamous Cell Carcinoma. Cancer Cell. 2018 May 14.Garrett M, Sperry J, Braas D, Yan W, Le TM, Mottahedeh J, Ludwig K, Eskin A, Qin Y, Levy R, Breunig JJ, Pajonk F, Graeber TG, Radu CG, Christofk H, Prins RM, Lai A, Liau LM, Coppola G, Kornblum HI. Metabolic characterization of isocitrate dehydrogenase (IDH) mutant and IDH wildtype gliomaspheres uncovers cell type-specific vulnerabilities. Cancer Metab. 2018 Apr 17;6:4. doi: 10.1186/s40170-018-0177-4.

2017

Nakano H, Minami I, Braas D, Pappoe H, Wu X, Sagadevan A, Vergnes L, Fu K, Morselli M, Dunham C, Ding X, Stieg AZ, Gimzewski JK, Pellegrini M, Clark PM, Reue K, Lusis AJ, Ribalet B, Kurdistani SK, Christofk H, Nakatsuji N, Nakano A. Glucose inhibits cardiac muscle maturation through nucleotide biosynthesis. Elife. 2017 Dec 12;6. pii: e29330. doi: 10.7554/eLife.29330.Flores A, Schell J, Krall AS, Jelinek D, Miranda M, Grigorian M, Braas D, White AC, Zhou JL, Graham NA, Graeber T, Seth P, Evseenko D, Coller HA, Rutter J, Christofk HR, Lowry WE. Lactate dehydrogenase activity drives hair follicle stem cell activation. Nat Cell Biol. 2017 Aug 14. doi: 10.1038/ncb3575.Schell JC, Wisidagama DR, Bensard C, Zhao H, Wei P, Tanner J, Flores A, Mohlman J, Sorensen LK, Earl CS, Olson KA, Miao R, Waller TC, Delker D, Kanth P, Jiang L, DeBerardinis RJ, Bronner MP, Li DY, Cox JE, Christofk HR, Lowry WE, Thummel CS, Rutter J. Control of intestinal stem cell function and proliferation by mitochondrial pyruvate metabolism. Nat Cell Biol. 2017 Aug 14. doi: 10.1038/ncb3593.Gu Y, Albuquerque CP, Braas D, Zhang W, Villa GR, Bi J, Ikegami S, Masui K, Gini B, Yang H, Gahman TC, Shiau AK, Cloughesy TF, Christofk HR, Zhou H, Guan KL, Mischel PS. mTORC2 Regulates Amino Acid Metabolism in Cancer by Phosphorylation of the Cystine-Glutamate Antiporter xCT. Mol Cell. 2017 Jun 22. pii: S1097-2765(17)30394-5.Krall AS, Christofk HR. Cell cycle: Division enzyme regulates metabolism. Nature. 2017 Jun 15;546(7658):357-358.Graham NA, Minasyan A, Lomova A, Cass A, Balanis NG, Friedman M, Chan S, Zhao S, Delgado A, Go J, Beck L, Hurtz C, Ng C, Qiao R, Ten Hoeve J, Palaskas N, Wu H, Müschen M, Multani AS, Port E, Larson SM, Schultz N, Braas D, Christofk HR, Mellinghoff IK, Graeber TG. Recurrent patterns of DNA copy number alterations in tumors reflect metabolic selection pressures. Mol Syst Biol. 2017 Feb 15;13(2):914.Momcilovic M, Bailey ST, Lee JT, Fishbein MC, Magyar C, Braas D, Graeber T, Jackson NJ, Czernin J, Emberley E, Gross M, Janes J, Mackinnon A, Pan A, Rodriguez M, Works M, Zhang W, Parlati F, Demo S, Garon E, Krysan K, Walser TC, Dubinett SM, Sadeghi S, Christofk HR, Shackelford DB. Targeted Inhibition of EGFR and Glutaminase Induces Metabolic Crisis in EGFR Mutant Lung Cancer. Cell Rep. 2017 .Jan 17;18(3):601-610. doi: 10.1016/j.celrep.2016.12.061.

2016

Gu W, Gaeta X, Sahakyan A, Chan AB, Hong CS, Kim R, Braas D, Plath K, Lowry WE, Christofk HR. Glycolytic Metabolism Plays a Functional Role in Regulating Human Pluripotent Stem Cell State. Cell Stem Cell. 2016.Krall AS, Xu S, Graeber TG, Braas D, Christofk HR. Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor. Nat Commun. 2016 Apr 29;7:11457. doi: 10.1038/ncomms11457.Hong CS, Graham NA, Gu W, Espindola Camacho C, Mah V, Maresh EL, Alavi M, Bagryanova L, Krotee PA, Gardner BK, Behbahan IS, Horvath S, Chia D, Mellinghoff IK, Hurvitz SA, Dubinett SM, Critchlow SE, Kurdistani SK, Goodglick L, Braas D, Graeber TG, Christofk HR. MCT1 Modulates Cancer Cell Pyruvate Export and Growth of Tumors that Co-express MCT1 and MCT4. Cell Rep. 2016 Feb 10. pii: S2211-1247(16)30035-3. doi: 10.1016/j.celrep.2016.01.057.

2015

Krall AS, Christofk HR. Rethinking glutamine addiction. Nat Cell Biol. 2015 Nov 27;17(12):1515-7.Thai M, Thaker SK, Feng J, Du Y, Hu H, Ting Wu T, Graeber TG, Braas D, Christofk HR. MYC-induced reprogramming of glutamine catabolism supports optimal virus replication. Nat Commun. 2015 Nov 12;6:8873. doi: 10.1038/ncomms9873.Fu X, Chin RM, Vergnes L, Hwang H, Deng G, Xing Y, Pai MY, Li S, Ta L, Fazlollahi F, Chen C, Prins RM, Teitell MA, Nathanson DA, Lai A, Faull KF, Jiang M, Clarke SG, Cloughesy TF, Graeber TG, Braas D, Christofk HR, Jung ME, Reue K, Huang J. 2-Hydroxyglutarate Inhibits ATP Synthase and mTOR Signaling. Cell Metab. 2015 Sep 1;22(3):508-15.Domigan CK, Warren CM, Antanesian V, Happel K, Ziyad S, Lee S, Krall A, Duan L, Torres-Collado AX, Castellani LW, Elashoff D, Christofk HR, van der Bliek AM, Potente M, Iruela-Arispe ML. Autocrine VEGF maintains endothelial survival through regulation of metabolism and autophagy. J Cell Science. 2015 Jun 15;128(12):2236-48.Drew BG, Hamidi H, Zhou Z, Villanueva CJ, Krum SA, Calkin AC, Parks BW, Ribas V, Kalajian NY, Phun J, Daraei P, Christofk HR, Hewitt SC, Korach KS, Tontonoz P, Lusis AJ, Slamon DJ, Hurvitz SA, Hevener AL. Estrogen Receptor (ER)α-regulated Lipocalin 2 Expression in Adipose Tissue Links Obesity with Breast Cancer Progression. J Biol Chem. 2015 Feb 27;290(9):5566-81.Sullivan WJ, Christofk HR. The metabolic milieu of metastases. Cell. 2015 Jan 29;160(3):363-4.

2014

Chin RM, Fu X, Pai MY, Vergnes L, Hwang H, Deng G, Diep S, Lomenick B, Meli VS, Monsalve GC, Hu E, Whelan SA, Wang JX, Jung G, Solis GM, Fazlollahi F, Kaweeteerawat C, Quach A, Nili M, Krall AS, Godwin HA, Chang HR, Faull KF, Guo F, Jiang M, Trauger SA, Saghatelian A, Braas D, Christofk HR, Clarke CF, Teitell MA, Petrascheck M, Reue K, Jung ME, Frand AR, Huang J. The metabolite α-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature. 2014 Jun 19;510(7505):397-401.Thai M, Graham NA, Braas D, Nehil M, Komisopoulou E, Kurdistani SK, McCormick F, Graeber TG, Christofk HR. Adenovirus E4ORF1-Induced MYC Activation Promotes Host Cell Anabolic Glucose Metabolism and Virus Replication. Cell Metab. 2014 Apr 1;19(4):694-701.Garon EB, Christofk HR, Hosmer W, Britten CD, Bahng A, Crabtree MJ, Hong CS, Kamranpour N, Pitts S, Kabbinavar F, Patel C, von Euw E, Black A, Michelakis ED, Dubinett SM, Slamon DJ. Dichloroacetate should be considered with platinum-based chemotherapy in hypoxic tumors rather than as a single agent in advanced non-small cell lung cancer. J Cancer Res Clin Oncol. 2014 Mar;140(3):443-52.

2013

Wang J, Hwang K, Braas D, Dooraghi A, Nathanson D, Campbell DO, Gu Y, Sandberg T, Mischel P, Radu C, Chatziioannou AF, Phelps ME, Christofk H, Heath JR. Fast metabolic response to drug intervention through analysis on a miniaturized, highly integrated molecular imaging system. J Nucl Med. 2013 Oct;54(10):1820-4.Babic I, Anderson ES, Tanaka K, Guo D, Masui K, Li B, Zhu S, Gu Y, Villa GR, Akhavan D, Nathanson D, Gini B, Mareninov S, Li R, Camacho CE, Kurdistani SK, Eskin A, Nelson SF, Yong WH, Cavenee WK, Cloughesy TF, Christofk HR, Black DL, Mischel PS. EGFR mutation-induced alternative splicing of Max contributes to growth of glycolytic tumors in brain cancer. Cell Metab. 2013 Jun 4;17(6):1000-8.Ahler E, Sullivan WJ, Cass A, Braas D, York AG, Bensinger SJ, Graeber TG, Christofk HR. Doxycycline alters metabolism and proliferation of human cell lines. PLoS One 2013 8(5):e64561.Krall AS, Christofk HR. Cancer: A metabolic metamorphosis. Nature 2013 496(7443):38-40.Williams KJ, Argus JP, Zhu Y, Wilks MQ, Marbois BN, York AG, Kidani Y, Pourzia AL, Akhavan D, Lisiero DN, Komisopoulou E, Henkin AH, Soto H, Chamberlain BT, Vergnes L, Jung ME, Torres JZ, Liau LM, Christofk HR, Prins RM, Mischel PS, Reue K, Graeber TG, Bensinger S. An essential requirement for the SCAP/SREBP signaling axis to protect cancer cells from lipotoxicity. Cancer Res. 2013 73(9):2850-62.Smith KB, Tran LM, Tam BM, Shurell EM, Li Y, Braas D, Tap WD, Christofk HR, Dry SM, Eilber FC, Wu H. Novel dedifferentiated liposarcoma xenograft models reveal PTEN down-regulation as a malignant signature and response to PI3K pathway inhibition. Am J Pathol. 2013 182(4):1400-11.McBrian MA, Saramipoor Behbahan I, Ferrari R, Su T, Huang TW, Li K, Hong CS,Christofk HR, Vogelauer M, Seligson DB, Kurdistani SK. Histone acetylation regulates intracellular pH. Mol Cell 2013 49(2):310-21.

2012

Braas D, Ahler E, Tam B, Nathanson D, Riedinger M, Benz MR, Smith KB, Eilber FC, Witte ON, Tap WD, Wu H, Christofk HR. Metabolomics strategy reveals subpopulation of liposarcomas sensitive to gemcitabine treatment. Cancer Discovery 2012 2(12):1109-17.Nagaraj R, Gururaja-Rao S, Jones KT, Slattery M, Negre N, Braas D, Christofk H, White KP, Mann R, Banerjee U. Control of mitochondrial structure and function by the Yorkie/YAP oncogenic pathway. Genes Dev. 2012 26(18):2027-37.Anastasiou D, Yu Y, Israelsen WJ, Jiang JK, Boxer MB, Hong BS, Tempel W, Dimov S, Shen M, Jha A, Yang H, Mattaini KR, Metallo CM, Fiske BP, Courtney KD, Malstrom S, Khan TM, Kung C, Skoumbourdis AP, Veith H, Southall N, Walsh MJ, Brimacombe KR, Leister W, Lunt SY, Johnson ZR, Yen KE, Kunii K, Davidson SM, Christofk HR, Austin CP, Inglese J, Harris MH, Asara JM, Stephanopoulos G, Salituro FG, Jin S, Dang L, Auld DS, Park HW, Cantley LC, Thomas CJ, Vander Heiden MG. Pyruvate kinase activators promote tetramer formation and suppress tumorigenesis. Nat Chem Biol. 2012 8(10): 839-47.Bensinger SJ, Christofk HR. New aspects of the Warburg effect in cancer cell biology. Semin Cell Dev Biol. 2012 23(4):353-61.

2011 & earlier

Vlashi E, Lagadec C, Vergnes L, Matsutani T, Masui K, Poulou M, Popescu R, Della Donna L, Evers P, Dekmezian C, Reue K, Christofk H, Mischel PS, Pajonk F. Metabolic state of glioma stem cells and nontumorigenic cells. Proc Natl Acad Sci U S A. 2011 108(38):16062-7.Christofk HR, Wu N, Cantley LC, Asara JM. Proteomic screening method for phosphopeptide motif binding proteins using Peptide libraries. J Proteome Res.2011 10(9), 44.Vander Heiden MG, Locasale JW, Swanson KD, Sharfi H, Heffron GJ, Amador-Noguez D, Christofk HR, Wagner G, Rabinowitz JD, Asara JM, Cantley LC (2010). Evidence for an alternative glycolytic pathway in rapidly proliferating cells. Science, 329(5998),1492-9.Vander Heiden Matthew G, Christofk Heather R, Schuman Eli, Subtelny Alexander O, Sharfi Hadar, Harlow Edward E, Xian Jun, Cantley Lewis C (2009). Identification of small molecule inhibitors of pyruvate kinase M2. Biochemical Pharmacology, 79(8), 1118-24.Asara John M, Christofk Heather R, Freimark Lisa M, Cantley Lewis C (2008). A label-free quantification method by MS/MS TIC compared to SILAC and spectral counting in a proteomics screen. Proteomics, 8(5), 994-9.Christofk Heather R, Vander Heiden Matthew G, Harris Marian H, Ramanathan Arvind, Gerszten Robert E, Wei Ru, Fleming Mark D, Schreiber Stuart L, Cantley Lewis C (2008). The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nature, 452(7184), 230-3.Christofk Heather R, Vander Heiden Matthew G, Wu Ning, Asara John M, Cantley Lewis C (2008). Pyruvate kinase M2 is a phosphotyrosine-binding protein. Nature, 452(7184), 181-6.Asara John M, Zhang Xiang, Zheng Bin, Christofk Heather H, Wu Ning, Cantley Lewis C (2006). In-Gel Stable-Isotope Labeling (ISIL): a strategy for mass spectrometry-based relative quantification. Journal of Proteome Research, 5(1), 155-63.Asara John M, Zhang Xiang, Zheng Bin, Maroney Lisa A, Christofk Heather R, Wu Ning, Cantley Lewis C (2006). In-gel stable isotope labeling for relative quantification using mass spectrometry. Nature Protocols, 1(1), 46-51.Wong Kelly A, Kim Rachel, Christofk Heather, Gao Jing, Lawson Gregory, Wu Hong (2004). Protein inhibitor of activated STAT Y (PIASy) and a splice variant lacking exon 6 enhance sumoylation but are not essential for embryogenesis and adult life. Molecular and Cellular Biology, 24(12), 5577-86.